At a minimum, the raw sequences must be filtered and the open reading frames located, translated, and aligned. Projects involving the discovery of novel proteins with new or improved functions usually accumulate large numbers of DNA sequences requiring many analysis steps.
XLibraryDisplay can be downloaded for free from /projects/xlibrarydisplay/. Though larger data sets can be handled, the program is best suited for analyzing approximately 10 000 or fewer leads or naïve clones which have been characterized using Sanger sequencing and other experimental approaches. Key steps for library characterization through lead discovery are available including library composition analysis, filtering by experimental data, graphing and correlating to experimental data, alignment to structural data extracted from PDB files, and generation of PyMOL visualization scripts. XLibraryDisplay accepts any DNA template which is used as a basis for trimming, filtering, translating, and aligning hundreds to thousands of sequences (raw, FASTA, or Phred PHD file formats).
XLibraryDisplay is an open source program integrated into Microsoft Excel for Windows that automates batch sequence processing via a simple step-by-step, menu-driven graphical user interface.
Also contained are step-by-step instructions for its use.Ĭlick the LandscapePages.docx file to begin your download.Protein engineering projects often amass numerous raw DNA sequences, but no readily available software combines sequence processing and activity correlation required for efficient lead identification. The landscape page contains a page number located in an area that is neither a part of the Header or Footer. It contains three pages and built in section breaks.
To make this all a bit simpler, a downloadable MS Word file has been created to further assist you. Whereas, Microsoft Word defines this layout as the landscape position.
The manual also makes reference to "horizontal" tables or figures. NOTE: The University of Iowa Graduate College Thesis Manual defines the portrait position as the "vertical position". The Graduate College requires students, writing their thesis/dissertation, to orient the page numbers in the portrait position and to be consistent, on portrait and landscape pages, throughout the entire document. Headers and footers are the most common place used to insert and position page numbers and are controlled by linking and unlinking the header/footer area for each section. Microsoft Word allows page orientation to be controlled by Section Breaks. In other words, the page number remains consistently portrait oriented even though all other items on the page may be presented with a landscape orientation. Remember, even though the item on the page is presented in a landscape orientation, bound books always locate the page number in the same place, facing the same direction for the reader. This is true even for a book which includes an intermittent page containing the occasional landscape oriented table or figure. Probably one of the most common requirements for publishing is to maintain consistency, in everything, throughout your work.Ĭonsistency in formatting also includes page number layout orientation (in other words, the direction the page number is facing). These guidelines are often specific to line spacing, page number placement, font specifics, and margin size. If you are writing something for future publication, you have most likely been given a set of standards that must be met first.